LOCUS       TGEX-HC-hG1[LALA        5828 bp    DNA     circular
DEFINITION  Human IgG1 TGEX Expression Vector (v7)
ACCESSION   
VERSION     .7
KEYWORDS    .
COMMENT     FT color
            FT join(5..585,607..611,1104..1127,3136..3724,3740..3838)
            FT /number="17"
            FT color
            FT join(1100..1103,1128..1131,5588..5616)
            FT /number="1"
            FT color
            FT 2753..3127
            FT /number="20"
            FT color
            FT join(1041..1099,1132..1475,1478,1481..1760,1763..2113,
            FT 2118..2123)
            FT /number="14"
            FT color
            FT 2114..2117
            FT /number="30"
            FT translated
            FT 2753..3127
            FT /frame="2"
            FT color
            FT 3912..4531
            FT /number="2"
            FT color
            FT join(2146..2156,2159..2658,2660,2663..2719)
            FT /number="7"
            FT color
            FT join(4686..4826,4828..5546)
            FT /number="10"
            FT color
            FT 612..1000
            FT /number="8"
FEATURES             Location/Qualifiers
     promoter        5..585
                     /label=CMV_Promoter
                     /color="1"
     misc_feature    603
                     /label=Start
     misc_feature    612..1000
                     /label=TPL
                     /note="including partial first intron with enhancer
                     region."
     primer_bind     974..995
                     /label=tgex-S3
     misc_binding    1019..1024
                     /db_xref="REBASE:EcoRI"
     CDS             1041..1103
                     /codon_start=1
                     /translation="METDTLLLWVLLLLAAQPAMA"
                     /gene
                     /label=pelBK
                     /note="Hybrid kappa/pelB leader peptide sequence"
     misc_binding    1085..1097
                     /db_xref="REBASE:SfiI"
     misc_binding    complement(1105..1110)
                     /db_xref="REBASE:BsaI"
     misc_binding    1113..1118
                     /db_xref="REBASE:MluI"
     misc_binding    1121..1126
                     /db_xref="REBASE:BsaI"
     CDS             1128..2120
                     /codon_start=1
                     /translation="ASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSG
                     ALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS
                     CDKTHTCPPCPAPEAAGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNW
                     YVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALGAPIEKT
                     ISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKT
                     TPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK*"
                     /label=huG1*01
     misc_feature    2118..2123
                     /gene
                     /label=Stop
                     /note
     misc_binding    2124..2129
                     /db_xref="REBASE:NheI"
     misc_feature    2146..2719
                     /gene
                     /label=IRES
                     /note
     primer_bind     complement(2192..2211)
                     /gene
                     /label=ires-R
                     /note
     misc_feature    2656..2662
                     /label=A7
                     /note
     misc_binding    2741..2746
                     /db_xref="REBASE:XbaI"
     misc_binding    2747..2752
                     /db_xref="REBASE:SalI"
     CDS             2753..3127
                     /codon_start=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /label=Zeo
                     /note
     misc_binding    3128..3135
                     /db_xref="REBASE:NotI"
     misc_feature    3136..3724
                     /gene
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element"
     polyA_signal    3740..3838
                     /label=BGpA
                     /note="rabbit beta-globin polyadenylation signal"
     misc_binding    3855..3860
                     /db_xref="REBASE:BglII"
     misc_binding    3861..3866
                     /db_xref="REBASE:HindIII"
     rep_origin      complement(3912..4531)
                     /label=pMB1
                     /gene="pBR322 origin"
     misc_difference complement(4368)
                     /label=T1034_mutation
                     /note="Lin-Chao 1992"
     CDS             complement(4686..5546)
                     /codon_start=1
                     /product="TEM1 beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /gene="bla"
                     /label=AmpR
                     /note="truncated"
     promoter        complement(5588..5616)
                     /label=AmpR_Promoter
     misc_binding    5679..5684
                     /db_xref="REBASE:BglII"
     misc_difference 1476..1481
                     /gene
                     /label=LALA
                     /note
     misc_difference 1761..1762
                     /gene
                     /label=P329G
                     /note
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatcct cactctcttc cgcatcgctg tctgcgaggg ccagctgttg gggtgagtac
      661 tccctctcaa aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag
      721 gatttgatat tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca
      781 gaaaagacaa tctttccttg atgatgtcat acttatcctg tccctttttt ttccacagct
      841 cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga aacccgtcgg
      901 cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac cggatcggaa
      961 aacctctcga gaaaggcgtc taaccagtca cagtcgcaag tttaaacgga tctctagcga
     1021 attcggcttg ggccgccacc atggagacag acacactcct gctatgggta ctgctgctct
     1081 tagcggccca gccggccatg gcaggagacc taacgcgtat ggtctcagca tccaccaagg
     1141 gcccatcggt cttccccctg gcaccctcct ccaagagcac ctctgggggc acagcggccc
     1201 tgggctgcct ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg
     1261 ccctgaccag cggcgtgcac accttcccgg ctgtcctaca gtcctcagga ctctactccc
     1321 tcagcagcgt ggtgaccgtg ccctccagca gcttgggcac ccagacctac atctgcaacg
     1381 tgaatcacaa gcccagcaac accaaggtgg acaagaaagt tgagcccaaa tcttgtgaca
     1441 aaactcacac atgcccaccg tgcccagcac ctgaagccgc ggggggaccg tcagtcttcc
     1501 tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg
     1561 tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg
     1621 tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg
     1681 tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca
     1741 aggtgtccaa caaagccctc ggagccccca tcgagaaaac catctccaaa gccaaagggc
     1801 agccccgaga accacaggtg tacaccctgc ccccatcccg ggatgagctg accaagaacc
     1861 aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg
     1921 agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg
     1981 gctccttctt cctctatagc aagctcaccg tggacaagag caggtggcag caggggaacg
     2041 tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacgcag aagagcctct
     2101 ccctgtctcc gggtaaataa acagctagcg gttccaacct tggttcccct ctccctttcc
     2161 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     2221 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     2281 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     2341 gaatgtcgtg aaggaagcag ttcctctggg agcttcttga agacaaacaa cgtctgtagc
     2401 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     2461 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     2521 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     2581 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     2641 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     2701 ttgaaaaaca cgatgataag cttgccacaa cccgggatcc tctagagtcg acatggccaa
     2761 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     2821 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     2881 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     2941 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     3001 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     3061 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cactttgtgg cagaggagca
     3121 ggactgagcg gccgcaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3181 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3241 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3301 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3361 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3421 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3481 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     3541 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3601 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3661 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3721 ccgcagatcc cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag
     3781 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg
     3841 tcgagatccc tcggagatct aagcttcatg tgagcaaaag gccagcaaaa ggccaggaac
     3901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4801 ggccccagtg ctgcaatgat accgcgtgac ccacgctcac cggctccaga tttatcagca
     4861 ataaaccagc cagccggaag cgccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4981 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5281 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5581 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5641 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaag atctatacat tgaatcaata
     5701 ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg gctattggcc
     5761 attgcatacg ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg
     5821 accgccat
//
